Murray made his long-awaited singles comeback last week at the Cincinnati Masters.
Richard Gasquet proved too much for the 32-year-old, who is still trying to build up match fitness on a singles court.
But there were promising signs from the three-time Grand Slam champion, who heads into this week aiming to continue his progress.
He has been drawn to face American Tennys Sandgren in the first round, with this being the first time the two have faced each other.
However, Murray has downplayed expectations of him achieving much in North Carolina.
“My expectations are very low,” he said.
“I’m hoping that by the end of this year, I can start getting back to the best that I’ll be.
“I just want to get through some matches and hopefully my body holds up well.”
Andy Murray vs Tennys Sandgren start time
Murray will be competing on Centre Court at the Wake Forest University this evening.
The day’s action gets underway at 8pm (BST) with Pablo Andujar taking on Nicolas Jarry first.
Steve Johnson vs Corentin Moutet is next up on Centre Court.
And Murray’s match against Sandgren is scheduled to take place not before 12am (BST).
Andy Murray vs Tennys Sandgren live stream
The Winston-Salem Open isn’t being televised on UK television, or on Amazon Prime like most of the US Open warm-up events.
The only way to watch the action will be to log in to either SkyBet or Bet365.
New customers can open an account by depositing a small amount, usually £5, to then stream the games live.