Federer makes his return to action this week for the first time since losing to Novak Djokovic in the Wimbledon final last month.
He has kept a low profile while putting in preparations for the US Open, but will be looking to make a big mark at the Cincinnati Masters this week.
The 38-year-old begins his tournament against Argentine Juan Ignacio Londero in what will be the first encounter between the two.
Londero already recorded an impressive win over Matteo Berrettini in the opening round of the Cincinnati Masters and reached the final of the Swedish Open last month.
His performances in 2019, which included his first ATP title at the Cordoba Open, has seen him climb to a career-high world ranking of 55.
But the 25-year-old faces a tough ask to get the better of Federer, who is aiming to win a record eighth title in Cincinnati.
Cincinnati is Federer’s most successful ATP Masters 1000 event, but he hasn’t won there since 2015.
Roger Federer vs Juan Ignacio Londero start time
The first round match between Federer and Londero is set to get underway at 12am BST.
Roger Federer vs Juan Ignacio Londero LIVE stream
Coverage of the Cincinnati Masters will be broadcast on Amazon Prime Video from August 11, Sunday.
Some of the action will also be available to watch on SkyBet, with Federer vs Londero set to get underway at 12am BST.
This year, Prime Video will broadcast on-site, live studio coverage across tournaments, including at the BNP Paribas Open from Indian Wells, the Fever Tree Championships from The Queens Club, the US Open, and the Nitto ATP Finals from The O2.
On-site field reporters will bring customers the latest action, analysis and commentary right from the court side at many tournaments.
In addition to 37 ATP Tour events and the US Open available exclusively on Prime Video with a Prime membership, Prime members in the UK who subscribe to Eurosport Player through Prime Video Channels for an additional £6.99 per month (following a 7-day free trial) will also benefit from full coverage of the French Open, and daily highlights and live coverage from the men’s and women’s finals of Wimbledon in 2019.